Should the low immunogenicity of human amniotic membrane be reconsidered in light of methodological advances in histocompatibility?
Lauriana Solecki1,2, Bastien Mathéaud1, Pauline Jamain3, Isabelle Jollet3, Christophe Picard4,5, Alain Coaquette6, Xavier Lafarge7,8, Florelle Gindraux2,9.
1Service d’ophtalmologie, CHU Besançon, University hospital of Besancon , Besançon, France; 2Laboratoire de Nanomédecine, Imagerie, Thérapeutique EA 4662 (LNIT), University of Franche-Comté, , Besancon , France; 3Laboratoire d’Histocompatibilité, Etablissement Français du Sang Nouvelle-Aquitaine, Poitiers, France; 4Laboratoire d’immunogénétique, Établissement français du sang PACA-Corse, Marseille, France; 5Aix-Marseille Université, CNRS, EFS, ADES UMR 7268, ,, Marseille, France; 6Service de virologie, CHU Besançon, Besançon, France; 7Laboratoire d’ingénierie tissulaire et cellulaire, Etablissement Français du Sang Nouvelle-Aquitaine, Bordeaux, France; 8INSERM U1035, Université de Bordeaux, Biothérapie des Maladies Génétiques Inflammatoires et Cancers , Bordeaux, France; 9Service de Chirurgie Maxillo-Faciale, Stomatologie et Odontologie Hospitalière, CHU Besançon, Besançon, France
Questions have been raised about immunogenicity after human amniotic membrane (hAM) transplantation. While hAM is said to have low immunogenicity, there is no recent clinical or laboratory evidence that hAM transplantation is safe from an immunological perspective. The first studies were done at the end of the 20th century. However, the techniques for detecting anti-human leukocyte antigen (HLA) antibodies have improved considerably over the last 20 years. Here we summarize the debate about hAM immunogenicity after transplantation and describe the methods we use to demonstrate the immunogenicity of an hAM graft, especially the presence of antibodies directed at HLA-G, whose expression is the hallmark of placenta.
We will report immunogenicity of hAM graft in two clinical indications using two innovative techniques.
Agence Biomédecine (France).
[1] Akle CA, Adinolfi M, Welsh KI, Leibowitz S, McColl I. Immunogenicity of human amniotic epithelial cells after transplantation into volunteers. Lancet 1981; 2: 1003–5.
[2] Kubo M, Sonoda Y, Muramatsu R, Usui M. Immunogenicity of Human Amniotic Membrane in Experimental Xenotransplantation. 2001; 42: 8.
[3] Houlihan JM, Biro PA, Harper HM, Jenkinson HJ, Holmes CH. The Human Amnion Is a Site of MHC Class lb Expression: Evidence for the Expression of HLA-E and HLA-G. The Journal of Immunology; : 11.
[4] Hammer A, Hutter H, Blaschitz A, et al. Amnion Epithelial Cells, in Contrast to Trophoblast Cells, Express All Classical HLA Class I Molecules Together With HLA-G. American Journal of Reproductive Immunology 1997; 37: 161–71.
