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Immune monitoring for rejection / Immunomodulation / tolerance

Saturday October 28, 2023 - 08:00 to 09:30

Room: Indigo 204

312.3 Proteomic Pathways in Rejected Grafts of Pig-to-Rhesus Corneal Xenotransplantation

Award Winner

Chung Young Kim, Korea has been granted the TTS-IXA Congress Scientific Award

Chung Young Kim, Korea

Clinical fellow
Department of Ophthalmology
Seoul National University Hospital

Abstract

Proteomic pathways in rejected grafts of pig-to-rhesus corneal xenotransplantation

Chung Young Kim1,2,3, Jae Won Oh4,5, Chang Ho Yoon1,2,3, Jin Suk Ryu1, Kwang Pyo Kim4,5, Chung-Gyu Park6,7, Mee Kum Kim1,2,3.

1Laboratory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea; 2Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea; 3Department of Ophthalmology, Seoul National University Hospital, Seoul, Korea; 4Department of Applied Chemistry, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin, Korea; 5Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul, Korea; 6Translational Xenotransplantation Research Center, Seoul National University College of Medicine, Seoul, Korea; 7Department of Microbiology and Immunology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea

Introduction Corneal xenotransplantation from pigs to non-human primates (NHPs) has shown promising long-term graft survival, but immune responses to xenogeneic antigens are still stronger than those to allogeneic antigens, even with immunosuppression. Thus, it is important to investigate major rejection pathways in corneal xenotransplantation. Although the alterations of proteomic pathways in allotransplant rejection have been investigated, to date, no analysis of such pathways in xenotransplant rejection has been reported. Therefore, this study aimed to investigate interactive immune pathways and their evolution with the progression of rejection by analyzing proteomics changes in rejected or rejection-ongoing corneal xenografts and aqueous humors in pig-to-NHP corneal xenotransplantation, even with immunosuppressive agents.

Methods Wild-type (WT) or α-1,3-galactosyltransferase gene-knockout (GTKO) porcine corneas were used as donor corneas. The NHPs with porcine corneal transplantation were divided into three to four groups depending on the permanent rejection, rejection-ongoing, survival, or donor types. The proteomes of corneal tissues and aqueous humor were analyzed with liquid chromatography-mass spectrometry. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) for pathways and protein–protein interaction network analysis were applied to estimate the biological functions of differentially expressed proteins (DEPs).

Results Among the 416 DEPs identified in the cornea, NF-κB1 and proteosomes (PSMD7, PSMA5, and PSMD3) were found to be related to xenogeneic graft rejection. In addition, oxidative phosphorylation and leukocyte activation-related pathways were found to play a role in rejection. Among the 66 DEPs identified in the aqueous humor, complement proteins (C3, C5, and C9) and cholesterol metabolic proteins (APOA1 and APOA2) were revealed to be related with xenogeneic rejection as biomarkers. Alternative pathways of the aqueous complement system were identified to be important in graft rejected or rejection-ongoing subjects. In the subgroup analysis, a few DEPs between WT and GTKO grafted groups were found, and Ig-like domain-containing proteins were the common DEPs.

Conclusion This analysis suggests that the key roles of NF-κB1, proteosomes, oxidative phosphorylation, and leukocyte activation-related inflammation in the graft, and the clinical relevance of aqueous complement pathways as a predictive biomarker of xenogeneic rejection in pig-to-NHP corneal xenotransplantation.

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI23C0923). .

References:

[1] Yoon CH, Choi HJ, Kim MK. Corneal Xenotransplantation: Where Are We Standing?. Prog Retin Eye Res. 2021 Jan;80:100876.
[2] Yoon CH, Choi SH, Choi HJ, Lee HJ, Kang HJ, Kim JM, et al. Long-Term Survival of Full-Thickness Corneal Xenografts From A1,3-Galactosyltransferase Gene-Knockout Miniature Pigs in Non-Human Primates. Xenotransplantation. 2020 27(1):e12559.
[3] Funding M, Vorum H, Honoré B, Nexø E, Ehlers N. Proteomic analysis of aqueous humour from patients with acute corneal rejection. Acta Ophthalmol Scand. 2005 Feb;83(1):31-9.

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